C7117
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 | Whole cell lysates were separated on SDS-PAGE and blotted with Anti-Cullin-1 (Cat. No. C7117). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and chemiluminescent substrate. Antibody dilution 1:1,000 Lanes 1. HEK293 cells (whole cell extract w/sample buffer) 2. HEK293 cells (RIPA lysate) 3. NIH3T3 cells (RIPA lysate) 4. Raji cells (RIPA lysate) 5, 6. HCT116 cells (RIPA lysate) 6. Inhibition of antibody binding with Cullin-1 immunizing peptide (human 759-776) (20 μg/mL) |
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C7117
Sigma
Anti-Cullin-1 antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-Cul1 |
Description
| Immunogen | synthetic peptide located at the C-terminus of human cullin-1 (amino acids 759-776). This sequence is identical in mouse cullin-1 and highly conserved (83% identity) in Drosophila cullin-1. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 85 kDa |
| species reactivity | human, mouse |
| application(s) | immunoblotting: 0.5-1 μg/mL using whole cell extract of human colon carcinoma HCT-116 cell line and whole cell extract of mouse fibroblasts NIH3T3 cell line |
| Gene Information | human ... CUL1(8454) |
| mouse ... Cul1(26965) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
Related Products
| Related product | C0371, Anti-Cullin-4A antibody produced in rabbit |
